1 x te buffer Search Results


lysis buffer 17  (R&D Systems)
95
R&D Systems lysis buffer 17
Lysis Buffer 17, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lysis buffer 17 - by Bioz Stars, 2026-03
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vwr extra pure  (Chem Impex International)
94
Chem Impex International vwr extra pure
Vwr Extra Pure, supplied by Chem Impex International, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
vwr extra pure - by Bioz Stars, 2026-03
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1x lysis buffer  (R&D Systems)
93
R&D Systems 1x lysis buffer
1x Lysis Buffer, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 93 stars, based on 1 article reviews
1x lysis buffer - by Bioz Stars, 2026-03
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ice cold lysis buffer 6  (R&D Systems)
92
R&D Systems ice cold lysis buffer 6
Ice Cold Lysis Buffer 6, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
ice cold lysis buffer 6 - by Bioz Stars, 2026-03
92/100 stars
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exosomes lysates  (R&D Systems)
91
R&D Systems exosomes lysates
Uptake of <t>exosomes</t> from high glucose-treated macrophages upregulates expression of inflammatory and fibrogenesis genes in fibroblasts. Characterization of exosomes from macrophage cell <t>line</t> <t>(RAW</t> 264.7 cells) by NanoSight dynamic light scattering analysis (A), transmission electron microscopy (B), and Western blotting and dot blot for exosome markers (C). Inset in panel B represents a closer view of the indicated region, scale bar, 100 μm. D, qRT-PCR data showing higher HuR mRNA levels in exosomes of high glucose-treated cells, compared with exosomes from normal glucose-treated cells (normalized to GAPDH, *P < .05). E, Western blot to show presence of HuR protein in macrophage exosome. F, Immunofluorescence staining showing uptake of PKH26-labeled macrophage exosomes (red) by fibroblasts (FB; NIH/3T3 fibroblast cell line; Flash Phalloidin green staining). DAPI to stain nuclei (blue); scale bar, 10 μm. G, qRT-PCR showing increase in mRNA expression of inflammatory and fibrogenesis-related genes in FB co-cultured with exosomes derived from high glucose-treated macrophages (Exo-HG), compared to FB treated with exosomes derived from normal glucose-treated macrophages (Exo-HG). Data normalized to β-actin expression. n = 3, *P < .01
Exosomes Lysates, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 91 stars, based on 1 article reviews
exosomes lysates - by Bioz Stars, 2026-03
91/100 stars
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edta buffer  (Teknova)
94
Teknova edta buffer
Uptake of <t>exosomes</t> from high glucose-treated macrophages upregulates expression of inflammatory and fibrogenesis genes in fibroblasts. Characterization of exosomes from macrophage cell <t>line</t> <t>(RAW</t> 264.7 cells) by NanoSight dynamic light scattering analysis (A), transmission electron microscopy (B), and Western blotting and dot blot for exosome markers (C). Inset in panel B represents a closer view of the indicated region, scale bar, 100 μm. D, qRT-PCR data showing higher HuR mRNA levels in exosomes of high glucose-treated cells, compared with exosomes from normal glucose-treated cells (normalized to GAPDH, *P < .05). E, Western blot to show presence of HuR protein in macrophage exosome. F, Immunofluorescence staining showing uptake of PKH26-labeled macrophage exosomes (red) by fibroblasts (FB; NIH/3T3 fibroblast cell line; Flash Phalloidin green staining). DAPI to stain nuclei (blue); scale bar, 10 μm. G, qRT-PCR showing increase in mRNA expression of inflammatory and fibrogenesis-related genes in FB co-cultured with exosomes derived from high glucose-treated macrophages (Exo-HG), compared to FB treated with exosomes derived from normal glucose-treated macrophages (Exo-HG). Data normalized to β-actin expression. n = 3, *P < .01
Edta Buffer, supplied by Teknova, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/edta buffer/product/Teknova
Average 94 stars, based on 1 article reviews
edta buffer - by Bioz Stars, 2026-03
94/100 stars
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lysis buffer 16  (R&D Systems)
91
R&D Systems lysis buffer 16
Uptake of <t>exosomes</t> from high glucose-treated macrophages upregulates expression of inflammatory and fibrogenesis genes in fibroblasts. Characterization of exosomes from macrophage cell <t>line</t> <t>(RAW</t> 264.7 cells) by NanoSight dynamic light scattering analysis (A), transmission electron microscopy (B), and Western blotting and dot blot for exosome markers (C). Inset in panel B represents a closer view of the indicated region, scale bar, 100 μm. D, qRT-PCR data showing higher HuR mRNA levels in exosomes of high glucose-treated cells, compared with exosomes from normal glucose-treated cells (normalized to GAPDH, *P < .05). E, Western blot to show presence of HuR protein in macrophage exosome. F, Immunofluorescence staining showing uptake of PKH26-labeled macrophage exosomes (red) by fibroblasts (FB; NIH/3T3 fibroblast cell line; Flash Phalloidin green staining). DAPI to stain nuclei (blue); scale bar, 10 μm. G, qRT-PCR showing increase in mRNA expression of inflammatory and fibrogenesis-related genes in FB co-cultured with exosomes derived from high glucose-treated macrophages (Exo-HG), compared to FB treated with exosomes derived from normal glucose-treated macrophages (Exo-HG). Data normalized to β-actin expression. n = 3, *P < .01
Lysis Buffer 16, supplied by R&D Systems, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lysis buffer 16/product/R&D Systems
Average 91 stars, based on 1 article reviews
lysis buffer 16 - by Bioz Stars, 2026-03
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lysis buffer 15  (R&D Systems)
90
R&D Systems lysis buffer 15
Uptake of <t>exosomes</t> from high glucose-treated macrophages upregulates expression of inflammatory and fibrogenesis genes in fibroblasts. Characterization of exosomes from macrophage cell <t>line</t> <t>(RAW</t> 264.7 cells) by NanoSight dynamic light scattering analysis (A), transmission electron microscopy (B), and Western blotting and dot blot for exosome markers (C). Inset in panel B represents a closer view of the indicated region, scale bar, 100 μm. D, qRT-PCR data showing higher HuR mRNA levels in exosomes of high glucose-treated cells, compared with exosomes from normal glucose-treated cells (normalized to GAPDH, *P < .05). E, Western blot to show presence of HuR protein in macrophage exosome. F, Immunofluorescence staining showing uptake of PKH26-labeled macrophage exosomes (red) by fibroblasts (FB; NIH/3T3 fibroblast cell line; Flash Phalloidin green staining). DAPI to stain nuclei (blue); scale bar, 10 μm. G, qRT-PCR showing increase in mRNA expression of inflammatory and fibrogenesis-related genes in FB co-cultured with exosomes derived from high glucose-treated macrophages (Exo-HG), compared to FB treated with exosomes derived from normal glucose-treated macrophages (Exo-HG). Data normalized to β-actin expression. n = 3, *P < .01
Lysis Buffer 15, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lysis buffer 15/product/R&D Systems
Average 90 stars, based on 1 article reviews
lysis buffer 15 - by Bioz Stars, 2026-03
90/100 stars
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lysis buffer  (R&D Systems)
93
R&D Systems lysis buffer
Uptake of <t>exosomes</t> from high glucose-treated macrophages upregulates expression of inflammatory and fibrogenesis genes in fibroblasts. Characterization of exosomes from macrophage cell <t>line</t> <t>(RAW</t> 264.7 cells) by NanoSight dynamic light scattering analysis (A), transmission electron microscopy (B), and Western blotting and dot blot for exosome markers (C). Inset in panel B represents a closer view of the indicated region, scale bar, 100 μm. D, qRT-PCR data showing higher HuR mRNA levels in exosomes of high glucose-treated cells, compared with exosomes from normal glucose-treated cells (normalized to GAPDH, *P < .05). E, Western blot to show presence of HuR protein in macrophage exosome. F, Immunofluorescence staining showing uptake of PKH26-labeled macrophage exosomes (red) by fibroblasts (FB; NIH/3T3 fibroblast cell line; Flash Phalloidin green staining). DAPI to stain nuclei (blue); scale bar, 10 μm. G, qRT-PCR showing increase in mRNA expression of inflammatory and fibrogenesis-related genes in FB co-cultured with exosomes derived from high glucose-treated macrophages (Exo-HG), compared to FB treated with exosomes derived from normal glucose-treated macrophages (Exo-HG). Data normalized to β-actin expression. n = 3, *P < .01
Lysis Buffer, supplied by R&D Systems, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/lysis buffer/product/R&D Systems
Average 93 stars, based on 1 article reviews
lysis buffer - by Bioz Stars, 2026-03
93/100 stars
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1x phosphate-buffered saline (pbs) nacalai tesque  (Nacalai)
90
Nacalai 1x phosphate-buffered saline (pbs) nacalai tesque
Uptake of <t>exosomes</t> from high glucose-treated macrophages upregulates expression of inflammatory and fibrogenesis genes in fibroblasts. Characterization of exosomes from macrophage cell <t>line</t> <t>(RAW</t> 264.7 cells) by NanoSight dynamic light scattering analysis (A), transmission electron microscopy (B), and Western blotting and dot blot for exosome markers (C). Inset in panel B represents a closer view of the indicated region, scale bar, 100 μm. D, qRT-PCR data showing higher HuR mRNA levels in exosomes of high glucose-treated cells, compared with exosomes from normal glucose-treated cells (normalized to GAPDH, *P < .05). E, Western blot to show presence of HuR protein in macrophage exosome. F, Immunofluorescence staining showing uptake of PKH26-labeled macrophage exosomes (red) by fibroblasts (FB; NIH/3T3 fibroblast cell line; Flash Phalloidin green staining). DAPI to stain nuclei (blue); scale bar, 10 μm. G, qRT-PCR showing increase in mRNA expression of inflammatory and fibrogenesis-related genes in FB co-cultured with exosomes derived from high glucose-treated macrophages (Exo-HG), compared to FB treated with exosomes derived from normal glucose-treated macrophages (Exo-HG). Data normalized to β-actin expression. n = 3, *P < .01
1x Phosphate Buffered Saline (Pbs) Nacalai Tesque, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1x phosphate-buffered saline (pbs) nacalai tesque/product/Nacalai
Average 90 stars, based on 1 article reviews
1x phosphate-buffered saline (pbs) nacalai tesque - by Bioz Stars, 2026-03
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nuclei isolation buffer 0.1% triton-x  (Promega)
90
Promega nuclei isolation buffer 0.1% triton-x
Uptake of <t>exosomes</t> from high glucose-treated macrophages upregulates expression of inflammatory and fibrogenesis genes in fibroblasts. Characterization of exosomes from macrophage cell <t>line</t> <t>(RAW</t> 264.7 cells) by NanoSight dynamic light scattering analysis (A), transmission electron microscopy (B), and Western blotting and dot blot for exosome markers (C). Inset in panel B represents a closer view of the indicated region, scale bar, 100 μm. D, qRT-PCR data showing higher HuR mRNA levels in exosomes of high glucose-treated cells, compared with exosomes from normal glucose-treated cells (normalized to GAPDH, *P < .05). E, Western blot to show presence of HuR protein in macrophage exosome. F, Immunofluorescence staining showing uptake of PKH26-labeled macrophage exosomes (red) by fibroblasts (FB; NIH/3T3 fibroblast cell line; Flash Phalloidin green staining). DAPI to stain nuclei (blue); scale bar, 10 μm. G, qRT-PCR showing increase in mRNA expression of inflammatory and fibrogenesis-related genes in FB co-cultured with exosomes derived from high glucose-treated macrophages (Exo-HG), compared to FB treated with exosomes derived from normal glucose-treated macrophages (Exo-HG). Data normalized to β-actin expression. n = 3, *P < .01
Nuclei Isolation Buffer 0.1% Triton X, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/nuclei isolation buffer 0.1% triton-x/product/Promega
Average 90 stars, based on 1 article reviews
nuclei isolation buffer 0.1% triton-x - by Bioz Stars, 2026-03
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Image Search Results


Uptake of exosomes from high glucose-treated macrophages upregulates expression of inflammatory and fibrogenesis genes in fibroblasts. Characterization of exosomes from macrophage cell line (RAW 264.7 cells) by NanoSight dynamic light scattering analysis (A), transmission electron microscopy (B), and Western blotting and dot blot for exosome markers (C). Inset in panel B represents a closer view of the indicated region, scale bar, 100 μm. D, qRT-PCR data showing higher HuR mRNA levels in exosomes of high glucose-treated cells, compared with exosomes from normal glucose-treated cells (normalized to GAPDH, *P < .05). E, Western blot to show presence of HuR protein in macrophage exosome. F, Immunofluorescence staining showing uptake of PKH26-labeled macrophage exosomes (red) by fibroblasts (FB; NIH/3T3 fibroblast cell line; Flash Phalloidin green staining). DAPI to stain nuclei (blue); scale bar, 10 μm. G, qRT-PCR showing increase in mRNA expression of inflammatory and fibrogenesis-related genes in FB co-cultured with exosomes derived from high glucose-treated macrophages (Exo-HG), compared to FB treated with exosomes derived from normal glucose-treated macrophages (Exo-HG). Data normalized to β-actin expression. n = 3, *P < .01

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Targeting exosome-associated human antigen R attenuates fibrosis and inflammation in diabetic heart

doi: 10.1096/fj.201901995R

Figure Lengend Snippet: Uptake of exosomes from high glucose-treated macrophages upregulates expression of inflammatory and fibrogenesis genes in fibroblasts. Characterization of exosomes from macrophage cell line (RAW 264.7 cells) by NanoSight dynamic light scattering analysis (A), transmission electron microscopy (B), and Western blotting and dot blot for exosome markers (C). Inset in panel B represents a closer view of the indicated region, scale bar, 100 μm. D, qRT-PCR data showing higher HuR mRNA levels in exosomes of high glucose-treated cells, compared with exosomes from normal glucose-treated cells (normalized to GAPDH, *P < .05). E, Western blot to show presence of HuR protein in macrophage exosome. F, Immunofluorescence staining showing uptake of PKH26-labeled macrophage exosomes (red) by fibroblasts (FB; NIH/3T3 fibroblast cell line; Flash Phalloidin green staining). DAPI to stain nuclei (blue); scale bar, 10 μm. G, qRT-PCR showing increase in mRNA expression of inflammatory and fibrogenesis-related genes in FB co-cultured with exosomes derived from high glucose-treated macrophages (Exo-HG), compared to FB treated with exosomes derived from normal glucose-treated macrophages (Exo-HG). Data normalized to β-actin expression. n = 3, *P < .01

Article Snippet: Protein extraction and Western blot analysis RAW 264.7 cells and exosomes lysates were prepared using cell lysis buffer 1 (Part No.: 890713, R & D Systems).

Techniques: Expressing, Transmission Assay, Electron Microscopy, Western Blot, Dot Blot, Quantitative RT-PCR, Immunofluorescence, Staining, Labeling, Cell Culture, Derivative Assay

Exosomes from high glucose-treated HuR-deficient macrophage abrogate inflammatory and fibrogenesis response in fibroblasts. Western blotting/densitometry analysis (A and B) and immunofluorescence staining (C) show efficient knockdown of HuR in mouse macrophage cell line (RAW 264.7) after 48 hours of lentiviral-based shRNA transduction. HuR KD, macrophage cells transduced with HuR-specific shRNA; WT, macrophage cells transduced with non-specific control shRNA. Scale bar, 20 μm. D, qRT-PCR analysis showing downregulation of mRNA related to inflammatory and/or fibrogenesis genes in fibroblasts (NIH3T3 cells) when co-cultured with exosomes from high glucose-treated HuR-deficient macrophage cells (HG-Exo-HGHuR KD). Data normalized to β-actin mRNA. (E and F) Western blot and densitometry analysis showing similar trend for MMP-9 and MMP13 proteins, data normalized to GAPDH. n = 3, *P < .01

Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Article Title: Targeting exosome-associated human antigen R attenuates fibrosis and inflammation in diabetic heart

doi: 10.1096/fj.201901995R

Figure Lengend Snippet: Exosomes from high glucose-treated HuR-deficient macrophage abrogate inflammatory and fibrogenesis response in fibroblasts. Western blotting/densitometry analysis (A and B) and immunofluorescence staining (C) show efficient knockdown of HuR in mouse macrophage cell line (RAW 264.7) after 48 hours of lentiviral-based shRNA transduction. HuR KD, macrophage cells transduced with HuR-specific shRNA; WT, macrophage cells transduced with non-specific control shRNA. Scale bar, 20 μm. D, qRT-PCR analysis showing downregulation of mRNA related to inflammatory and/or fibrogenesis genes in fibroblasts (NIH3T3 cells) when co-cultured with exosomes from high glucose-treated HuR-deficient macrophage cells (HG-Exo-HGHuR KD). Data normalized to β-actin mRNA. (E and F) Western blot and densitometry analysis showing similar trend for MMP-9 and MMP13 proteins, data normalized to GAPDH. n = 3, *P < .01

Article Snippet: Protein extraction and Western blot analysis RAW 264.7 cells and exosomes lysates were prepared using cell lysis buffer 1 (Part No.: 890713, R & D Systems).

Techniques: Western Blot, Immunofluorescence, Staining, Knockdown, shRNA, Transduction, Control, Quantitative RT-PCR, Cell Culture